Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 24: Posters - Single Molecule Biophysics
BP 24.2: Poster
Montag, 7. März 2016, 17:30–19:30, Poster C
Direct observation of the intermolecular association of PGL-3, a liquid protein droplet component. — •Jose A. Morin1,2, Shambaditya Saha3, Anthony Hyman3, and Stephan Grill2,3 — 1Max Plank Institute for the Physics of Complex Systems, Nöthnitzer Str. 38, 01187 Dresden, Germany — 2Biotechnology Center, Technical University, Tatzberg 47, 01307 Dresden, Germany — 3Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany
P granules, the germ line determinants in C. elegans, are an excellent example of spatiotemporal cytoplasmatic organization. During the first cell division these non-membranous organelles undergo a liquid-liquid phase separation and become localized to the posterior half of the cell. It have been shown that PGL-3, a key component of P granules, is capable of phase separation by it self into a liquid phase in the test tube. In this work we address the molecular causes for liquid formation in protein systems. We have stablished a single molecule experimental assay to measure the interaction energy between individual PGL-3 proteins. Using the manipulation capabilities of our dual trap optical tweezers, a single molecule force spectroscopy technique, we place two PGL-3 coated beads into close proximity to accurately measure the interaction kinetics between a small number of PGL-3 proteins. Our data exhibit a rich kinetic behavior, where transient interactions can be clearly distinguished from the background and therefore the frequency and time duration of this interactions accessed. Moreover, a cooperative association between these proteins can be hinted.