Regensburg 2016 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 28: Systems Biology & Gene Expression and Signalling
BP 28.7: Vortrag
Dienstag, 8. März 2016, 12:00–12:15, H44
Stochasticity in DNA Replication of Archaea — •Jens Karschau1, Ulrik Günther2,3, and Alessandro de Moura4 — 1MPI PKS, Dresden, Germany — 2MPI CBG, Dresden, Germany — 3TU Dresden, Dresden, Germany — 4University of Aberdeen, Aberdeen, UK
DNA is the building plan of every living organism, which is contained in either a linear or circular chromosome. Replicating this chromosome begins from origins—the starting points from which DNA–copying forks emerge. We previously showed for the linear case that finite ends set optimal configurations for origins to give fast overall replication time. This depends on their probability to activate as well as their relative distance to another [1].
Here, we discuss replication on a circular chromosome: where forks never stop at any ends, walk around the circle, and finally coalesce with another. The process bears similarity to a nucleation and growth process on a ring—as is in bacteria and archaea, with the latter carrying multiple origins. On the one hand, we again show that the optimal location of origins strongly depends on origin distance as well as their activation probability in conditions allowing for the chromosome to be copied only once. On the other hand, under favourable conditions, simultaneous re–replication with clustered origins in nearby groups actually minimises chromosomal duplication times. We relate our findings to published experimental data to distinguish between settings for optimal growth of an archaeal species.
[1] J Karschau, JJ Blow, APS de Moura, PRL, 2012.