Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 43: Cell Adhesion
BP 43.5: Vortrag
Mittwoch, 9. März 2016, 16:15–16:30, H43
Morpho-dynamics and Mechanics of T lymphocytes — Pierre Dillard1,2, Astrid Wahl1, Fuwei Pi1, Ranime Allameddine1, Emmanuelle Benard1, Pierre-Henri Puech2, Anne Charrier1, Laurent Limozin2, and •Kheya Sengupta1 — 1CINaM/AMU-CNRS UMR 7325, Marseille, France. — 2LAI/INSERM UMR 1067 AMU-CNRS UMR 7333, Marseille, France.
We investigate adhesion and membrane organization of T lymphocytes interacting with surrogate antigen presenting cells (sAPCs) carrying the ligand anti-CD3 against the T cell receptor (TCR) complex. The sAPCs comprise supported bilayers with mobile/immobilized ligands (BiophysJ 2014), or ordered arrays of ligand nano-dots in a non-adhesive matrix (NanoLett 2013,2015), or soft elastomers. We show that ligand mobility is an important control parameter in cell spreading: cells adhere but fail to spread on mobile ligands, spreading can be rescued by suppressing myosin activity. We also demonstrate a dual scale of T cell response: locally, the cell responds at the nano-scale and restructures its membrane according to local cues; globally, it integrates the signal and responds to an average dose. Finally, the mechano-response of T cells is very different from connective tissue cells: unlike most previously reported cell types, T-cells spread more on soft than on hard elastomers. These results taken together point to original aspects of TCR-mediated response to mechanical cues which are should be relevant for understanding lymphocyte mechanotransduction.