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BP: Fachverband Biologische Physik
BP 48: Posters - Bioimaging and Spectroscopy
BP 48.11: Poster
Wednesday, March 9, 2016, 17:00–19:00, Poster C
Single Shot Quantitative Phase Microscopy Technique — •Tobias Neckernuss1, Xiaoming Jiang1,2, Jonas Pfeil1, Christoph Koch2, and Othmar Marti1 — 1Institute of Experimental Physics, Ulm University, Germany — 2Department of Physics, Humboldt University Berlin, Germany
The thickness of adherent cells is an important parameter in various biological and biophysical applications. However it is a difficult task to measure cell thickness with a microscope when using conventional microscopy techniques. There are several commercial systems around, measuring the optical path length through the cell with an interferometric approach. However, most of these systems use a compact Mach-Zehnder interferometer which is difficult to customize for special needs and additional instrumentation. To overcome this, we designed a setup that allows us to measure the optical path length through a transparent phase object with an inline holographic approach. We use a two camera system with two slightly different focus points. The sample is illuminated by a LED light source with sufficient spatial coherence. For reconstruction of the phase image the "Transport of Intensity Equation" is used for a first guess and an iterative algorithm for phase retrieval refines the reconstruction by forward and backward propagation of the reconstructed images and comparison to the captured ones. With this method we are able to determine the phase shift during transmission through a phase object. The main advantage of this compared to conventional z-stack techniques is the potential for high acquisition speeds.