Regensburg 2016 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 48: Posters - Bioimaging and Spectroscopy

BP 48.12: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Rotational Dark-Field Microscope for Fast Image Acquisition — •Daniel Geiger, Tobias Neckernuß, and Othmar Marti — Institute of Experimental Physics, Ulm University

The technique to obtain label-free optical images beyond the diffraction limit has been published recently [1]. Incoherent addition of coherent scattering images illuminated from different directions leads to an increase in contrast between two adjacent objects. However, this original setup is, due to the implementation with a spatial light modulator, only able to take images at rates around 1 Hz.

We replaced the spatial light modulator with a rotating double prism setup that can rotate with much higher frequencies. Our setup is designed to operate at 100 Hz that is the maximum image capturing rate of the used camera. Furthermore the illumination angle can be dynamically adjusted between zero and maximum angle given by the used objective's NA by variation of the prism separation. In principle, this setup can be enhanced to rotation rates in the kHz regime by using a hollow shaft electromotor that contains the prisms. This allows the label-free observation of fast biological processes on a length scale that was so far not accessible by conventional light microscopy.

[1] P. von Olshausen and A. Rohrbach, Opt. Lett. 38, 4066-4069 (2013)