Regensburg 2016 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 48: Posters - Bioimaging and Spectroscopy

BP 48.13: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Light sheet microscopy using Bessel beams and the STED principle — •Luis Köbele, Cristian Gohn-Kreuz, and Alexander Rohrbach — University of Freiburg, Laboratory for Bio- and Nano-Photonics, 79110 Freiburg, Germany

Light sheet microscopy is an imaging technique which features enhanced optical sectioning by using a thin, sheet-like illumination of only that part of the object, which is in the plane of focus. By the use of computer-generated holograms, we generate self-reconstructing Bessel beams, which showed enhanced propagation stability and penetration depth in scattering media and are thus superior, particularly for imaging in dense biological specimen. However, the pronounced ring system, which facilitates the self-reconstructing property of the scanned Bessel beams, produces a significant image background by exciting out-of-focus fluorophores. By superposing a concentrically aligned, doughnut shaped Bessel beam, stimulated emission depletion (STED) is used to improve the light sheet quality, generating effectively thinner light sheets with reduced background. We present first results of such an imaging setup and discuss advantages and challenges.