Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 48: Posters - Bioimaging and Spectroscopy

BP 48.14: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Photothermal detection of single gold nanoparticles in living fibroblasts — •Alice Abend, Romy Schachoff, and Frank Cichos — Universität Leipzig, Linnéstr. 5, 04103 Leipzig, Germany

Live cell bioimaging allows for the observation of cellular processes and their dynamics and provides insight into functions of cells such as metabolism, replication and movement. Modern nanotechnology enables manufacturing of nanometer sized objects with tailored optical properties and specific functionalization which turns them into ideal optical probes for several imaging techniques. Our method to transfer nanoscopic objects into the cytoplasm of live mammalian cells is called mechanodelivery [1]. This approach is based on mechanical damage of the cell membrane to deliver nano objects into the intracellular space. In contrast to passive delivery strategies allows the mechanodelivery approach for deposition of nano objects without them being engulfed in vesicles. We deliver gold nanoparticles (AuNPs) to the cells as they are photostable and allow for long-term imaging and seem to be less toxic to living organisms in comparison to semiconductor quantum dots. Our imaging method, photothermal optical microscopy, provides sensitive detection of AuNPs and is therefore suitable to prove the presence of AuNPs in the fibroblasts' interior. As photothermal microscopy is based on heating of the contrast agent, AuNPs can double as heat sources for inducing local intracellular temperature fields which could be useful to manipulate cellular functions such as protein synthesis and metabolism processes.

[1] Nanoscale, 2014, 6, 4538