Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 48: Posters - Bioimaging and Spectroscopy

BP 48.7: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Measurement of the height of living cells via an optimized phase-shifting interferometer. — •Shuaishuai Li¹, Xia Wang², and Othmar Marti¹ — ¹Institute for Experimental Physics, University of Ulm, 89069 Ulm, Germany — ²School of Optoelectronics, Beijing Institute of Technology, 100081, China

Phase-Shifting Interference Microscopy is a real-time observation technique. It is widely used for observing the growth process of monolayer cells and has many advantages, such as high precision, non-contact measurement etc. According to the principle of phase-shifting interferometry measuring method, a dual-angle incident interferometer is designed in our lab. Using a CCD digital imaging device to record the vision field, we can get a serial of cell interference patterns with certain phase changes. Phase unwrapping is the step that we extract the phase of an interference pattern. Afterward, we could reductive the Wavefront Pattern which can reflect the microscopic appearance and calculate the height value of living cells. We synthesize the advantages of the branch-cut method and the quality guide method. Experimental results show that this fusion phase unwrapping method is effective and practical in the processing of cell interferograms.Finally, through the experiment setup we designed, we measured the height value of one Styrene particles, which had been accurately measured by ZYGO measure instrument. The relative error is 0.023.