Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 48: Posters - Bioimaging and Spectroscopy

BP 48.8: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Application of Rotational Dark-Field Microscopy in Biology — •Daniela Beck, Daniel Geiger, Tobias Neckernuß, and Othmar Marti — Institute of Experimental Physics, Ulm University

It has been published recently, that rayleigh's criterion can be beaten by total internal reflection dark-field imaging [1]. This is based on the incoherent summation of images that are obtained by coherent scattering at different angles of incidence. The original setup is altered in order to increase the maximum possible frame rate.

Our new measurement technique sets the path for high temporal and increased spatial resolution. This makes the observation of biological processes on the millisecond timescale with increased resolution compared to Abbe's limit feasible. Preliminary measurements on biological samples show the prospects of this measurement setup that is also capable of simultaneous fluorescence and bright-field imaging.

[1] P. von Olshausen and A. Rohrbach, Opt. Lett. 38, 4066-4069 (2013)