Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 49: Posters - Cell Mechanics and Migration & Physics of Cancer

BP 49.20: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Investigations on Single Squamous Cell Carcinoma Cells — •Susanne Steeger¹, Julia Kristin², Marcel Glaas², Jörg Schipper², and Mathias Getzlaff¹ — ¹Heinrich-Heine-Universität Düsseldorf, Deutschland — ²Univ.-HNO-Klinik Düsseldorf, Deutschland

In this contribution we report on measurements of the mechanoelastic properties of ENT squamous cell carcinoma cells. The study of these single cancer cells in culture medium is carried out by Atomic Force Microscopy. Our main interest is the determination oft the Youngs Modulus calculated by the Hertzian Model. We identify the elasticity of cancer cells in order to compare it with that of similar benign cells. Because Live Cell Imaging is a challenging task we first focus on testing different cantilevers and various strategies to treat the cells carefully. Just recently we apply our new AFM with QI-Mode (JPK NanoWizard 3) which allows for a more detailed investigation of living cells. In order to determine the individual properties of the cancer cells we additionally analyse their cytoskeleton (actin and tubulin) by using a confocal fluorescence microscope. Cancer cells are known for their modified cytosekelton which is reflected in the different elasticities of both cancer and comparable benign cells.