Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 49: Posters - Cell Mechanics and Migration & Physics of Cancer

BP 49.7: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Mechano-sensitivity is cell type specific — •Galina Kudryasheva and Florian Rehfeldt — Georg-August-Universität Göttingen Fakultät für Physik III. Physikalisches Institut

Nowadays it is widely acknowledged that cellular function, morphology and fate are dependent on the mechanical properties of their micro-environment. Human mesenchymal stem cells (hMSCs) are a striking example that stem cell differentiation into various cell types can be guided by tuning the extracellular matrix stiffness. While the entire differentiation process can take several days up to weeks, the structure and dynamics of stress fibers can be used as an early morphological marker and theoretically modeled using classical mechanics with an active spring model. We use this approach to analyze the mechanical cell-matrix interactions of hMSCs and several types of differentiated cells, such as C2C12 myoblasts , SAOS-2 osteoblasts, human primary osteoblasts and 3T3 fibroblasts. We plate hMSCs and differentiated cells on elastic poly-acrylamide hydrogels covering the whole physiological range of stiffness given by Young's moduli E from 1 to 130 kPa. Applying immunofluorescence approach we label stress fibers and analyze cytoskeletal morphology by fluorescence microscopy. We analyze cell shape and extract corresponding material constants that show distinct differences during the differentiation process in different cell types. Our experiments showed that cellular susceptibility to the substrate elasticity is highly cell type specific.