Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 49: Posters - Cell Mechanics and Migration & Physics of Cancer

BP 49.8: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Mechanical properties of young and senescence dermal fibroblast cells using passive microrheology. — •Samira Khalaji¹, Fenneke KleinJan¹, Eugenia Makrantonaki², Vida Farsam², Ulla Nolte¹, Karin Scharffetter-Kochanek², and Kay-E Gottschalk¹ — ¹Institut für Experimentelle Physik, Universität Ulm — ²Klinik für Dermatologie und Allergologie, Universitätsklinikum Ulm

Biological aging is a multi-dimensional process that takes place over a whole range of scales from the nanoscopic alterations within cells, over transformations in tissues and oragans. On the single cell level, aging involves in gene mutations, altered gene expression and post translational modifications of proteins. A variety of proteins are affected, including proteins of the cell cytoskeleton. Previous work quantified the gene and protein expression of cytoskeleton proteins in senescent and young fibroblasts. Their results show that senescent skin fibroblasts have an upregulated expression of the intermediate filament (IF) protein vimentin in contrast to actin and tubulin which are downregulated. IFs play an important role in providing mechanical stability of cells. However the mechanical properties of IFs depending on cellular senescence or age of the donor has not been studied so far. Hence, we employed passive microrheology on young and senescence human dermal fibroblasts from donors with different age and different population doubling level. In contrast to the expectations, our primary results show no significant differences in the viscoelastic properties of fibroblasts depending on age of the donor or cellular replicative senescence.