Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 50: Posters - Cell Adhesion

BP 50.2: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Single cell force spectroscopy and initial cell adhesion — •Hendrik Brehme, Philipp Wysotzki, Marco Stubbe und Jan Gimsa — Universität Rostock, Lehrstuhl für Biophysik, Gertrudenstraße 11a, D-18057 Rostock, Deutschland

For medical implants, there is a general risk for so-called "implant-associated infections". The starting point for a possible infection is the "race for the surface", in which bacteria and body cells compete in making initial contacts to the implant's surface. Bacteria which may successfully colonize the surface tend to form biofilms, which can hardly be fought by the immune system of the patient. In the experiments, the individual behavior of single cells during their initial adhesion was investigated using single cell force spectroscopy. For this, cells of the bone cell line MC3T3 or bacteria were attached to the cantilever and their initial adhesion behavior to different surfaces was measured using a NanoWizard II atomic force microscope (JPK, Berlin).

The setpoint and adhesion forces were correlated with the electric impedance of a microelectrode structure for single cell detection (single cell-interdigitating electrode structure; SC-IDES).