Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 51: Posters - Cytosceletal Filaments

BP 51.1: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Cytoskeletal reorganization in human platelets during spreading — •Aishwarya Paknikar, Gerrit Brehm, Tim Dullweber, and Sarah Köster — Institute for X-Ray Physics, Georg-August-Universität Göttingen, Germany

To maintain the life-sustaining process of repairing damaged blood vessels, activated platelets change their shape, adhere, spread and contract in a hemostatic plug to seal the injuries. Dynamic and ordered rearrangements of their acto-myosin and microtubule (MT) cytoskeleton are responsible for these processes. We image the actin and MTs while they remodel in platelets in a time-resolved manner by labeling them with the recently introduced SiR-actin/tubulin probes. We demonstrate the ability to directly observe the formation of F-actin structures and coiling of the MTs as the platelets spread. The averaged actin intensity of single platelets reveals an initial steep rise followed by a linear increase that gradually reaches a plateau indicating the formation and increase in content of polymerized actin until the platelet spreads completely. By treatment of the platelets with pharmacological inhibitors we can indirectly show the crosstalk between the acto-myosin and microtubule dynamics. Our real-time cytoskeletal dynamics are all in agreement to post-fixation literature studies. Our results highlight a novel approach for studying real-time platelet cytoskeleton dynamics which is an important step in understanding the structural and mechanical aspects of platelet function better.