Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 51: Posters - Cytosceletal Filaments
BP 51.2: Poster
Mittwoch, 9. März 2016, 17:00–19:00, Poster C
Molecular assembly studied in microflow by fluorescence correlation spectroscopy — •Viktor Schroeder1, Eleonora Perego1, Harald Herrmann2, and Sarah Köster1 — 1Institute for X-Ray Physics, Georg-August-Universität Göttingen, Germany — 2Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany
We present a combination of microfluidic diffusive mixing and fluorescence correlation spectroscopy (FCS) to study rapid molecular assembly processes. In FCS, information about diffusing fluorescent particles is retrieved by analyzing the correlation of intensity fluctuations. To overcome the limited temporal resolution of FCS caused by long measuring times on the order of at least ten seconds, we use continuous flow microfluidic tools to map the temporal evolution to a spatial axis. Thus we achieve a temporal resolution of milliseconds with a dead time of only one second. Molecular assembly processes are initiated by the inflow of trigger molecules. The macromolecules of interest then spread over the whole cross section of the channel via diffusion, thus leading to a constant concentration downstream. Data are collected at different positions along the channel. As an example, we employ this method for studying the assembly of vimentin intermediate filament protein.