Regensburg 2016 – scientific programme
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BP: Fachverband Biologische Physik
BP 57: Membranes and Vesicles I
BP 57.10: Talk
Thursday, March 10, 2016, 12:30–12:45, H43
Standing-Wave X-Ray Fluorescence Enables Near-Angstrom Precision Localization of Biologically Important Chemical Elements in Molecular Layers — •Emanuel Schneck1, Ernesto Scoppola2, 3, Jakub Drnec4, Cristian Mocuta5, Roberto Felici4, Dmitri Novikov6, Giovanna Fragneto2, and Jean Daillant5 — 1Max Planck Institute of Colloids and Interfaces, Potsdam, Germany — 2Institut Laue-Langevin, Grenoble, France — 3Institut de Chimie Séparative de Marcoule, France — 4European Synchrotron Radiation Facility, Grenoble, France — 5Synchrotron Soleil, Gif-sur-Yvette, France — 6Deutsches Elektronen-Synchrotron, Hamburg, Germany
In nature, biomolecules are often organized as functional thin layers in interfacial geometries. The most prominent examples are the biological membranes. But biomolecular layers also play important roles in context with biotechnological surfaces, for instance when they are the result of adsorption processes. For the understanding of many biological or biotechnologically relevant processes, detailed structural insight into the involved biomolecular layers is required. Here, we use standing-wave x-ray fluorescence to determine element-specific density profiles in solid-supported lipid and protein monolayers with near-Angstrom resolution. The technique complements traditional reflectometry experiments which merely yield the layers' "global" density profiles. While earlier work mostly focused on relatively heavy elements, typically ions, we also localize the comparatively light elements S and P, which are found in many biomolecules and therefore particularly interesting.