Regensburg 2016 – scientific programme

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BP: Fachverband Biologische Physik

BP 57: Membranes and Vesicles I

BP 57.6: Talk

Thursday, March 10, 2016, 11:30–11:45, H43

Fast tracking of single molecules in live cell membranes at ultra-high resolution with interferometric scattering microscopy (iSCAT) — •Richard Taylor and Vahid Sandoghdar — Max Planck Institute for the Science of Light, Erlangen, Germany

Conventional approaches to track diffusion of lipids and proteins in membranes use fluorescence microscopy, but a low fluorescence rate and inevitable photobleaching severely limit the localisation precision on both the short and long timescales. Furthermore, fluorescence microscopy traditionally also suffers from limited axial resolution. We report on the use of interferometric scattering microscopy (iSCAT) for monitoring of a gold nanoparticle-labelled protein within the live HeLa cell membrane. iSCAT particle tracking has previously demonstrated accurate tracking of labeled single lipids within synthetic membranes to nanometric precision at fast millisecond framerates [1]. Here, we demonstrate use of iSCAT microscopy to track the diffusion of gold labeled transmembrane proteins within the live HeLa cell membrane. We show that one may track the probe diffusion both in- and out-of-plane, with nanometer-level accuracy and microsecond framerates.

C.-L. Hsieh, S. Spindler, J. Ehrig, V. Sandoghdar, J. Phys. Chem. B. 118, 1545-1554, (2014).