Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 58: Cytoskeletal Filaments

BP 58.10: Vortrag

Donnerstag, 10. März 2016, 12:30–12:45, H44

Structure and formation dynamics of stress fibers in adult stem cells — •Carina Wollnik¹, Benjamin Eltzner², Stephan Huckemann², and Florian Rehfeldt¹ — ¹Third Institute of Physics - Biophysics, Georg-August-University, Göttingen, Germany — ²Institute for Mathematical Stochastics, Georg-August-University, Göttingen, Germany

During differentiation, pluripotent adult human mesenchymal stem cells (hMSCs) become various cell types like nerve, bone or muscle precursor cells. Here, substrate stiffness is sufficient to guide hMSCs towards different lineages in the absence of additional biochemical stimuli [1]. Key players are stress fibres that generate and transmit contractile forces throughout the cell [3] and mediate cell-matrix mechanics. Characteristic reorganisation of stress fibres is detected within 24 hours and can be used as early morphological marker [2]. Using massive parallel life-cell imaging of RFP-Lifeact transfected hMSCs on substrates of different stiffness during early stem cell differentiation, we detect distinct pattern formation strategies of stress fibres, traced with novel sophisticated tracking algorithms [4,5]. [1] A. Engler et al., Cell (2006) [2] A. Zemel et al., Nature Physics (2010) [3]E. K. Paluch et al, BMC Biology (2015) [4] B. Eltzner et al., PLoS One (2015); [5] S. Huckemann et al., Bernoulli (2015) - to appear;