Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 60: Pattern Formation (Joint Session with DY)
BP 60.10: Vortrag
Donnerstag, 10. März 2016, 12:15–12:30, H46
Cyclosis-mediated transfer of reactive oxygen species and its relation to the formation of pH bands in Chara alga cells — •Alexey Eremin1, Ralf Stannarius1, Anna Komarova2, and Alexander Bulychev2 — 1Institute of Experimental Physics, Otto von Guericke University Magdeburg — 2Faculty of Biology, Lomonosov Moscow State University, 119992 Moscow, Russia
In internodal cells of characean algae, cyclosis participates in formation of light-dependent patterns of surface pH and photosynthetic activity. Hydrogen peroxide, being a signalling molecule and a stress factor, is known to accumulate under excessive irradiance. In our study, we explore the spatio-temporal dynamics of pH-band formation on the surfaces of internodal Chara corallina cells and study the kinetics of H2O2 production in chloroplasts and its release into the cytoplasm under local illumination. We demonstrate that in cells exhibiting active streaming, H2O2 first accumulates in the illuminated region and then enters into the streaming cytoplasm. The results suggest that H2O2 released from chloroplasts is transported along the cell with the cytoplasmic flow. It is proposed that the shift of cytoplasmic redox poise and light-induced elevation of cytoplasmic pH facilitate the opening of H+/OH-permeable channels in the plasma membrane.