Regensburg 2016 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 13: Poster: Nanoparticles, Nanocrystals and Composites

CPP 13.11: Poster

Montag, 7. März 2016, 18:15–21:00, Poster B2

Highly efficient energy transfer from quantum dot to allophycocyanin in hybrid structures — •Anastasia Karpulevich^1,3, Evgeny Maksimov¹, Nikolay Sluchanko², Alexandr Vasiliev¹, and Vladimir Paschenko¹ — ¹Lomonosov Moscow State University, 119991, Moscow, Russia — ²A.N.Bach Institute of Biochemistry, 119071, Moscow, Russia — ³Institute of Physical Chemistry, Hamburg University, 20146, Hamburg, Germany

Development of light-energy converters based on photosynthetic mechanisms is one of the promising directions in modern biophysics. Quantum dots can be used as a substitution to the native light-harvesting antennae, increasing absorbtion cross-section of purified photosystem as a result of non-radiative energy transfer [1]. We investigated model system consisting of CdSe/ZnS core-shell quantum dot (QD) and protein allophycocyanin (APC). Using spectroscopic methods we shown that QD-APC hybrid systems are able to self-assemble in solution. We found that steric accessibility plays a key role for the energy transfer: the energy migration efficiency in hybrid structures reaches 90 % for APC monomers and only 30 % for trimers. Additionally, we obtained stable covalently bound QD-APC hybrids. We observed considerable amplification of APC fluorescence in the optical range of excitation between 400 and 500 nm, where QD absorption prevails. The results might be used for the creation of bright and stable fluorescent dyes based on allophycocyanin.

[1] E.G. Maksimov et al., Biochem., vol. 77, no. 6, pp. 624*630 (2012).