Regensburg 2016 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 44: Poster: Biomaterials and Biopolymers

CPP 44.1: Poster

Mittwoch, 9. März 2016, 18:15–21:00, Poster B2

Macromolecular HPMA-Based drug delivery system - behavior in protein environment — •Xiaohan Zhang¹, Bart-Jan Niebuur¹, Petr Chytil², Tomas Etrych², Sergey K. Filippov², Alexey Kikhney³, Florian Wieland³, Dmitri I. Svergun³, and Christine M. Papadakis¹ — ¹Technische Universität München, Physik-Department, Fachgebiet Physik weicher Materie, Garching, Germany — ²Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic — ³European Molecular Biology Laboratory, DESY, Hamburg, Germany

Polymer drug carriers based on N-(2-Hydroxypropyl)methacrylamide (HPMA) copolymers bearing cholesterol moieties have been studied extensively over the past few years, to understand the aggregation behavior and shape of the copolymers in dilute aqueous solutions [1,2]. Above certain concentration, micelle-like nanoparticles (NPs) having ellipsoidal shape are observed. However, there is a lack of knowledge on the behavior of these drug carriers in human blood environment.

We use fluorescence life-time correlation spectroscopy (FLCS) and small angle X-ray scattering (SAXS) to investigate HPMA copolymers that are dissolved in an aqueous solution of human serum albumin (HSA) to reveal the interaction between the NPs and HSA. The size of the NPs is reduced when HSA is present. We suspect that some of the cholesterol moieties bind to HSA and are removed from the nanoparticles.

[1] S. K. Filippov, et al., Biomacromolecules, 2012, 13, 2594

[2] S. K. Filippov, et al., Biomacromolecules, 2013, 14, 4061