Regensburg 2016 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 59: Biomaterials and Biopolymers III (joint session BP/CPP/MM, organized by BP)

CPP 59.2: Vortrag

Donnerstag, 10. März 2016, 15:15–15:30, H45

Multiple bio-functionalization in 3D-scaffolds for cell manipulation realized by orthogonal (photo)chemistry — •Vincent Hahn¹, Benjamin Richter², Tanja Claus^3,4, Guillaume Delaittre^3,5, Christopher Barner-Kowollik^3,4, Martin Wegener^1,6, and Martin Bastmeyer² — ¹Institute of Applied Physics, Karlsruhe Institute of Technology (KIT) — ²Zoological Institute and Institute for Functional Interfaces, KIT — ³Institute for Chemical Technology and Polymer Chemistry, KIT — ⁴Institute for Biological Interfaces, KIT — ⁵Institute for Toxicology and Genetics, KIT — ⁶Institute of Nanotechnology, KIT

In recent years, we have applied Direct Laser Writing to fabricate 3D-microscaffolds for culturing cells in a well-defined environment and investigated cellular responses, e.g., contractility, adhesion and shape.

By sequential writing of different photoresists, patterned scaffolds are realized. They consist of protein-binding polymers next to regions containing light-activatable monomers in a non-protein binding background. Upon light-activation we were able to biotinylate specific regions in the passivating backbone. When incubated with a protein solution, proteins adsorb only onto protein-binding polymer areas. The biotin-linker is subsequently addressed by using avidin and any other biotinylated protein of choice. This technique has been successfully applied to fabricate scaffolds functionalized with two different adhesion proteins that selectively direct cell adhesion.

Such scaffolds might prove useful for applications in tissue engineering and stem cell differentiation.