Regensburg 2016 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 8: Hydrogels and Microgels
CPP 8.7: Vortrag
Montag, 7. März 2016, 17:00–17:15, H40
Superresolved fluorescence and electron microscopy to visualize the core-shell structure of microgels — •Dominik Wöll1, Arjan Gelissen1, Alex Oppermann1, Tobias Caumanns2, Pascal Hebbeker1, Rahul Tiwari3, Joachim Mayer2, Walter Richtering1, and Andreas Walther3 — 1Institute of Physical Chemistry, RWTH Aachen University, Landoltweg 2, 52056 Aachen, Germany. — 2GFE Central Facility for Electron Microscopy, RWTH Aachen University, Mies-van-der-Rohe-Straße 59, 52074 Aachen, Germany. — 3DWI - Leibniz-Institute for Interactive Materials, Forckenbeckstr. 50, 52074 Aachen, Germany
Microgels are an interesting class of soft matter materials with properties depending on their internal structure. Electron and superresolved fluorescence microscopy will be presented as methods to open up the possibility to visualize the core-shell structure of single microgels. In both cases, appropriate labelling conditions had to be chosen to gain sufficient contrast. As the approaches used so far cannot distinguish between different axial (z-)position, the obtained images consist of 2D projections of the 3D positions of labels. From these projections, we developed a method to calculate the 3D radial distribution function using appropriate core-shell models established in microgel research.