Regensburg 2016 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 33: Photonics and Nanooptics I: Infrared Spectroscopy
O 33.6: Vortrag
Dienstag, 8. März 2016, 11:45–12:00, H4
Surface-enhanced infrared spectroscopy for poly-peptide sensing on single nanostructures — •Rostyslav Semenyshyn1, Frank Neubrech1, Mario Hentschel1, Xinghui Yin1, Jochen Vogt2, Felix Weiher1, Christoph Stanglmair3, Claudia Pacholski3, and Harald Giessen1 — 14th Physics Institute and Research Center SCoPE, University of Stuttgart — 2Kirchhoff Institute for Physics, University of Heidelberg — 3Max Planck Institute for Intelligent Systems, Stuttgart
Metal nanowires and nanoslits with plasmon resonances tuned to the infrared spectral region are ideally suited for surface enhanced infrared absorption (SEIRA) spectroscopy. Since its discovery, SEIRA has been applied to chemical analysis and biochemical sensing.
In our present work, we will demonstrate how SEIRA is used for the detection of peptides and proteins in minute amounts, and sensing of temperature-induced structural changes. For this purpose, nanostructures were functionalized by mercaptoundecanoic acid in order to selectively bind poly-L-lysine to the gold surface. Additionally, we covered other nanoantennas with specially designed collagen peptides. The plasmonic resonances of such nanostructures were tuned to the amide I and II bands of the respective poly-peptides. A tailored flow cell was used to allow the control of molecular adsorption on the nanostructures and in-situ SEIRA in aqueous solutions. Following this approach, the spectroscopic detection of about 13000 poly-peptides on one single slit could be achieved. In further experiments, structural changes of the poly-peptides due to temperature changes were observed.