Dresden 2017 – scientific programme

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BP: Fachverband Biologische Physik

BP 10: Posters - Single Molecule Biophysics

BP 10.1: Poster

Monday, March 20, 2017, 17:30–19:30, P3

Single molecule detection in microflow — •Eleonora Perego, Viktor Schroeder, and Sarah Köster — Institute for X-Ray Physics, Georg-August-Universität Göttingen, Germany

In recent years single molecule fluorescence methods have emerged as powerful method to study assembly, aggregation and diffusion of biomolecules. We use techniques like fluorescence correlation spectroscopy (FCS) or photon counting histogram (PCH) to study these process with high spatial resolution. However these techniques are inherently "slow". Thus in order to overcome this limitation, we combine these methods with microfluidics. This allow us to study the assembly and the diffusion of biomolecules in a time-resolves manner. One application is the study of early time points of protein assembly, like intermediate filaments, that are usually difficult to measure with more classical bulk experiments. Moreover, by employing our setup, it is possible to identify the passage of a single molecule through the excitation volume by tuning the concentration and the flow conditions of the sample stream. Our results show that the combination of microfluidic and single molecule fluorescence methods provides a very suitable approach for studying the aggregation of biomolecules in real time, which is important for understanding cellular behavior.