Dresden 2017 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 10: Posters - Single Molecule Biophysics
BP 10.3: Poster
Montag, 20. März 2017, 17:30–19:30, P3
Label-free iSCAT detection of secretory proteins from single living cells — •Andre Gemeinhardt1, Matthew McDonald1, Katharina König1, Stefanie Schaller2, Michael Aigner2, Andreas Mackensen2, and Vahid Sandoghdar1 — 1Max Planck Institute for the Science of Light, Erlangen, Germany — 2Department of Haematology and Oncology, Universitätsklinikum Erlangen, Germany
Interaction and communication between cells is partly guided via an exchange of different proteins. Here, we report the detection of single unlabeled proteins secreted by individual living cells. This is realized via an interferometric scattering technique (iSCAT) developed in our laboratory that circumvents the need to fluorescently label the biomolecules under study. The technique’s use of interference between a small scattering signal and a larger reference wave paves the way to detect even the smallest particles. We apply this method to analyze the secretomics of single cytotoxic T-cells under external stimulation. By varying the system between body temperature and fever-like conditions, we observe a clear trend towards shorter reaction times after triggering cytokine secretion. The developed method is a promising way to elucidate the complicated nature of immune response through the basic principles of membrane-mediated protein release and cellular exchange.