Dresden 2017 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 10: Posters - Single Molecule Biophysics
BP 10.6: Poster
Montag, 20. März 2017, 17:30–19:30, P3
Catch bond interaction between cell surface sulfatase Sulf2 and glycosaminoglycans — Ahmet Erol1, •Sören Grannemann1, Christian Bartz2, Volker Walhorn1, Thomas Dierks2, and Dario Anselmetti1 — 1Experimental Biophysics & Applied Nanoscience, Bielefeld University, Germany — 2Biochemistry I, Bielefeld University, Germany
In biological adhesion, the biophysical mechanism of specific non-covalent biomolecular interaction can be divided in slip- and catch-bonds, respectively. Conceptually, slip bonds exhibit a reduced bond lifetime under increased external force and catch-bonds, in contrast, an increased lifetime for a certain force interval. Catch bonds therefore act in a counter intuitive manner.
Upon investigating the specific interaction between the unique hydrophilic domain (HD) of the human cell-surface sulfatase Sulf2 against its native glycosaminoglycan (GAG) target substrate heparan sulfate (HS) by single-molecule force spectroscopy (SMFS), we found clear evidence of catch-bond behavior which, by means of control experiments, could be specifically related to the GAG 6-O-sulfation site. Alongside to our previous work on the catch bond behavior of HDSulf1 [1], we analyzed the data within the theoretical framework of a force mediated transition between two coupled slip bond regimes.
[1] A. Harder et al., Biophysical Journal 108(7), p1709-1717 (2015)