Dresden 2017 – scientific programme
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BP: Fachverband Biologische Physik
BP 13: Bioimaging and Spectroscopy II
BP 13.3: Talk
Tuesday, March 21, 2017, 10:15–10:30, HÜL 386
Brillouin Microscopy: A non-invasive way of studying elasticity in biological tissue — •Dmitry Richter1,2, Alba Diz-Muñoz1, and Robert Prevedel1 — 1European Molecular Biology Laboratory, Heidelberg, Germany — 2Heidelberg University, Heidelberg, Germany
In order to map the elastic properties of cells and tissues, a spatial resolution in the order of the diffraction limit and access to internal structures is required. Up until now elastography has employed insufficient methods; techniques such as atomic-force microscopy require mechanical contact or invasion, giving no information about the internal structure. On the other hand, macroscopic methods like ultrasound imaging are unable to resolve cellular/subcellular components.
A possible alternative is Brillouin spectroscopy. It is based on the inelastic scattering of light on thermally induced, low frequency phonons, enabling a non-contact, high resolution study of elastic properties [1, 2]. Here, we discuss Brillouin microscopy as an imaging tool, highlighting its potential applications in biology as well as its inherent challenges and limitations. Moreover we show preliminary experimental results on single cells and multicellular organisms.
[1] G. Scarcelli, S. H. Yun, Nature photonics (2008)
[2] F. Palombo et al, Analyst (2014)