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BP: Fachverband Biologische Physik

BP 13: Bioimaging and Spectroscopy II

BP 13.5: Vortrag

Dienstag, 21. März 2017, 10:45–11:00, HÜL 386

Investigations of squamous cell carcinoma cells and dysplastic oral keratinocytes — •Maja Strugačevac¹, Susanne Steeger¹, Jan Lietz¹, Julia Kristin², Marcel Glaas², Jörg Schipper², and Mathias Getzlaff¹ — ¹Institute of Applied Physics, Heinrich-Heine-Universität Düsseldorf — ²Hals-Nasen-Ohrenklinik, Universitätsklinikum Düsseldorf

The aim of this study is to develop new, alternative, cell-selective treatment strategies for squamous cell carcinoma of the head-neck area. As one of first steps mechano-elastic properties of oral carcinoma cells (HNSCC) were examined using Atomic Force Microscopy (AFM). The Young's Modulus calculated by the Hertzian Model was determined in order to quantify the elasticity of the cells. As known the differences between elasticities of carcinoma and benign cells can - in part - be attributed to the modified cytoskeleton of cancer cells.

Squamous cell carcinoma cells as well as oral keratinocytes are examined by confocal fluorescence microscopy in order to obtain more information about physical cell properties. Different markers allow us to highlight specific cell organelles including cell membranes and cytoskeleton. This contribution focuses on staining optimization and investigation of differences between both cell lines.