Dresden 2017 – scientific programme

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BP: Fachverband Biologische Physik

BP 16: Biotechnology and Bioengineering

BP 16.2: Talk

Tuesday, March 21, 2017, 12:00–12:15, HÜL 386

Micropatterning of reagent-free, high energy crosslinked gelatin hydrogels for bioapplications — •Astrid Weidt¹, Benedikt Heyart², Emilia Wisotzki^1,3, Mareike Zink¹, and S.G. Mayr^3,4 — ¹Nachwuchsgruppe Biotechnologie und Biomedizin, Soft Matter Physics Division, Universität Leipzig, Germany — ²Institut für Biochemie und Biotechnologie, Technische Universität Braunschweig, Germany — ³Leibniz Institut für Oberflächenmodifizierung (IOM) e.V., Leipzig, Germany — ⁴Division of Surface Physics, Universität Leipzig, Germany

The development of biocompatible materials that support the regeneration of soft tissues is of high importance for medical applications. Materials such as collagen and gelatin show a great potential as graft materials and cell carriers, since they originate from the extracellular matrix. Adequate structuring of hydrogels as cellular substrates is mandatory for successful cell adhesion. Here, a reagent-free method for crosslinking and subsequent micropatterning of gelatin hydrogels was demonstrated. The simple and effective method of micromolding was employed to transfer structures in the micrometer range during electron irradiation onto gelatin. Thermally-stable substrates were fabricated, characterized by regular grooves with widths of 3.75 to 170 *m and depths of several hundred nanometers. We show that the microstructured hydrogels promote cell adhesion and contact guidance of NIH 3T3 mouse embryonic fibroblasts. Cells attached and adapted on the surfaces. Changes to the cell morphology were observed within 4 day in culture under physiological conditions.