Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 2: Bioimaging and Spectroscopy I

BP 2.10: Vortrag

Montag, 20. März 2017, 12:30–12:45, HÜL 386

High Resolution Imaging of Cellular Signaling Using Fluorescent Nanosensor Arrays — •Sebastian Kruss — Institut für Physikalische Chemie, Universität Göttingen

Intercellular communication via chemical signaling proceeds with both spatial and temporal components, but analytical tools, such as microfabricated electrodes, have been limited to just a few probes per cell. In this work, we use a non-photobleaching fluorescent nanosensor array based on single walled carbon nanotubes (SWCNT) rendered selective to the neurotransmitter dopamine to study its release from neuroprogenitor cells at a resolution exceeding 20,000 sensors/cell. This allows the spatial and temporal dynamics of dopamine release, following stimulation, to be measured at exceedingly high spatiotemporal resolution. We observe localized, unlabeled release sites of dopamine spanning 100 ms to seconds that correlate with negative membrane curvature, and not predominately the positive curvature associated with the tips of cellular protrusions as intuitively expected. The results illustrate how directionality of chemical signaling is shaped by membrane morphology, and highlight the advantages of nanosensor arrays that can provide high spatial and temporal resolution of chemical signaling.