Dresden 2017 – scientific programme

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BP: Fachverband Biologische Physik

BP 2: Bioimaging and Spectroscopy I

BP 2.6: Talk

Monday, March 20, 2017, 11:30–11:45, HÜL 386

Disentangling the effects of viscosity and refractive index mismatch in single-focus FCS — •Jonas Mücksch, Petra Schwille, and Eugene P. Petrov — Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany

Fluorescence Correlation Spectroscopy (FCS) is a powerful tool to assess molecular mobilities in various settings ranging from bulk solutions to model lipid membranes, to living cells. The media in which FCS measurements are performed, frequently differ from water with respect to their viscosity and their refractive index. On the other hand, it has been shown that the refractive index mismatch can severely affect the results of FCS measurements [1]. Here, we explore under which conditions it is possible to disentangle the effects of the solution viscosity and refractive index mismatch on FCS measurements carried out using a standard confocal microscope-based setup and thus employ this experimental technique for reliable determination of diffusion coefficients in various media.

[1] J. Enderlein, I. Gregor, D. Patra, T. Dertinger, and U. B. Kaupp, ChemPhysChem 6, 2324 (2005).