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BP: Fachverband Biologische Physik

BP 24: Posters - Protein Structure and Dynamics

BP 24.4: Poster

Dienstag, 21. März 2017, 14:00–16:00, P1A

Biophysical characterization of integrin alpha IIb-beta3 under physiological and stress conditions — •Una Janke, Peter Nestler, and Mihaela Delcea — University of Greifswald, ZIK HIKE, Fleischmannstr. 42, 17498 Greifswald, Germany

The heterodimeric transmembrane platelet receptor integrin alpha IIb-beta 3 (αIIbβ3) plays a crucial role in haemostasis and is involved in the autoimmune disease Immune Thrombocytopenia (ITP). ITP patients develop a higher bleeding risk due to autoantibody mediated platelet destruction. The immunogenicity (i.e. capacity of the immune system to induce an immune response) depends strongly on the conformation of the αIIbβ3. Thus, we aim to study the influence of closed, opened and intermediate conformations on the binding properties of αIIbβ3 to ITP patient autoantibodies. Here we present a strategy to investigate the effect of mutations, external stress factors and binding partners on the αIIbβ3 conformation. The interaction under physiological conditions, e.g. in a membrane environment, is investigated using surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) after incorporating αIIbβ3 into liposomes. Different incorporation protocols are compared with regards to liposome-αIIbβ3 conjunction. First results indicate that pH, manganese ions and temperature are important influence factors.