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BP: Fachverband Biologische Physik

BP 25: Posters - Cytoskeletal Filaments

BP 25.1: Poster

Dienstag, 21. März 2017, 14:00–16:00, P2-EG

Investigations of the cytoskeleton of squamous cell carcinoma cells and oral keratinocytes — •Nina Bartels¹, Maja Strugacevac¹, Susanne Steeger¹, Jan Lietz¹, Julia Kristin², Marcel Glaas², Jörg Schipper², and Mathias Getzlaff¹ — ¹Institute of Applied Physics, Heinrich-Heine-Universität Düsseldorf, Deutschland — ²Hals-Nasen-Ohrenklinik, Universitätsklinikum Düsseldorf, Deutschland

In order to investigate differences between oral carcinoma cells (HNSCC) and oral keratinocytes (DOK) two different techniques, Atomic Force Microscopy (AFM) and fluorescence microscopy, were used.

By means of AFM the mechano-elastic properties of carcinoma cells were investigated, because a possible cause for the differences between HNSCC and DOK cells are modifications in the cytoskeleton of cancer cells. To calculate the elasticity of the cells, the Young's Modulus was determined using the Hertzian Model.

The cytoskeleton of HNSCC and DOK was examined using confocal fluorescence microscopy. This contribution is focused on the comparison of the cytoskeleton and its staining for the two cell lines. For the staining of the cytoskeleton, SiR-actin and SiR-tubulin were used. An optimized staining process for both, actin and tubulin, was found for the collective staining of HNSCC and DOK.