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BP: Fachverband Biologische Physik

BP 26: Posters - Cell Adhesion

BP 26.5: Poster

Dienstag, 21. März 2017, 14:00–16:00, P2-EG

Mechanosensitivity of Murine Kidney Epithelial Cells — •Theresa Hoppe and Florian Rehfeldt — Third Institute of Physics, University of Göttingen

Recent experiments have shown that cell adhesion and subsequent spreading is dictated by the elasticity of the underlying substrate. Additionally, the ligand density and type of ligand employed result in differences in a cell's spreading behavior.

Here we investigate cellular spreading on two different types of collagen (Collagen I and IV) and by way of using two cell types. Collagen I is a major component of the connective tissue extracellular matrix (ECM), whereas collagen IV is primarily prominent in the ECM of epithelial tissue cells. Previous studies have well established optimal ligand concentrations of Collagen I for hMSC's. In this study, we determined the optimal collagen IV concentration for the maximal spreading of human mesenchymal stem cells (hMSC) on 10kPa polyacrylamide (PA) gels. We also studied the adherence of primary murine tubular epithelial cells on PA gels of varying elasticity coated with the same concentrations of collagen I and IV as for the hMSCs. We analyzed cell spreading via fluorescence microscopy.

Results indicate that on comparison with collagen I hMSC's spread area is lower on collagen IV for the same 10kPa PA gels. Surprisingly murine cell adherence seems to be ligand specific. On varying substrate stiffness, collagen I promotes spreading of these cells whereas collagen IV failed to supply sufficient adhesion sites.