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BP: Fachverband Biologische Physik

BP 26: Posters - Cell Adhesion

BP 26.6: Poster

Dienstag, 21. März 2017, 14:00–16:00, P2-EG

Measuring Cell Dynamics at the Substrate-Interface with Surface Plasmon Resonance Miscroscopy — •Eva Kreysing, Hossein Hassani, and Andreas Offenhäusser — ICS8/PGI8, Forschungszentrum Juelich, 52425 Juelich

In neuroelectronics the cell-electrode distance is one of the most critical parameters during cell recordings. Cardiomyocyte-like cells are among the most popular model systems because they periodically generate an action potential. This feature also leads to a cell contraction which affects the cell-electrode distance. To achieve a qualitative and quantitative characterization of the dynamics at the interface in vitro and lable-free, we built a surface plasmon resonance microscope (SPRM). Using gold coated sapphire chips as the substrate for cell culture it is possible to excite plasmons in the gold layer due to specific illumination. The resonance frequency of the plasmons depends strongly upon the dielectric constant of the gold's environment. In turn the angle spectrum of the reflected light depends upon said resonance frequency. Due to these dependencies it is possible to deduce the cell-substrate distance. Our microscope is capable of imaging the interface in a live-imaging mode where we can observe cell dynamics qualitatively. A scanning mode uses localized surface plasmons to measure the cell-substrate distance. The resolution in z-direction lies in the nanometer range. This allows us to measure the movement of the cell membrane at each scanning point with a time resolution of 150 ms. Using this method we have been able to record the dynamics of multiple cardiomyocytes.