Dresden 2017 – scientific programme
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BP: Fachverband Biologische Physik
BP 32: Posters - DNA/RNA
BP 32.1: Poster
Tuesday, March 21, 2017, 14:00–16:00, P2-OG1
Condensation of DNA Brush Networks — •Günther Pardatscher1, Dan Bracha2, Shirley S. Daube2, Ohad Vonshak2, Roy H. Bar-Ziv2, and Friedrich C. Simmel1,3 — 1TU München, Garching, Germany — 2Weizmann Institute of Science, Rehovot, Israel — 3Nanosystems Initiative Munich, München, Germany
DNA condensation via interaction with multivalent salts or histones is known for the regulation of genes and metabolism, and for the generation of self-assembling rod-like, spheroidal or toroidal DNA nanostructures. We here investigated the condensation of e-beam patterned, surface-bound DNA brushes into arbitrarily shaped DNA bundles. DNA molecules of 1 micron length (3 kbp) were immobilized on lines as thin as 100 nm in width, before condensation was induced by addition of spermidine. Starting at a nucleation site, DNA condensates grew via an inverted domino effect by adsorbing neighboring DNA chains.
The confinement of DNA brushes to widths below the contour length of the DNA resulted in changes in condensation dynamics and condensate morphology from two-dimensional dendritic to a single, straight one-dimensional DNA bundle. In contrast to condensation from solution or extended 2D brushes, 1D DNA bundles can be guided along predesigned, arbitrary pathways, while persisting over tens of micrometers. We further applied the process in unconventional approaches to computational problems, e.g. in determining possible solutions for a maze.