Dresden 2017 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 38: Membranes and Vesicles I
BP 38.11: Vortrag
Mittwoch, 22. März 2017, 12:45–13:00, HÜL 386
DNA-oligomers as model likers for membrane adhesion — •Mohammad Kamal1, Franck Thibaudau1, Ana-Sunčana Smith2, and Kheya Sengupta1 — 1Centre Interdisciplinaire de Nanoscience de Marseille (CINaM), Aix - Marseille Université-CNRS UMR 7325, Campus de Luminy, Case 913, 13288 Marseille Cedex 9, France — 2PULS Group, Department of Physics and Cluster of Excellence: EAM, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany
In the last decade, DNA-linkers have been extensively used as selective glue to tune inter-colloidal interactions with the aim of creating new meta-materials which exploit the versatility of DNA. Here we take a similar approach to use the power of DNA origami to test ideas in the context of model membrane adhesion. We use Reflection Interference Contrast Microscopy (RICM) to quantify the interaction between the membrane of a giant unilamellar vesicle and a supported lipid bilayer employing short DNA sequences as linkers. The linker-length was varied independently of its biding affinity. The linker flexibility was also varied by using either single (ss) or double stranded (ds) DNA. For ds-DNA, the separation (d) between the two interacting membranes is smaller than or equal to the linker length. For corresponding ss-DNA, d is always smaller. The linker-length influences the adhesion strength only at low concentrations, and for a given length, ds-DNA is a more efficient binder than ss-DNA. We also explore the possibility of adhesion induced phase separation using binary-mixtures of linkers. The results are interpreted in terms of a thermodynamic model.