Dresden 2017 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 38: Membranes and Vesicles I
BP 38.3: Vortrag
Mittwoch, 22. März 2017, 10:15–10:30, HÜL 386
Shape remodeling vesicles by localized actin polymerization — •Katharina Henneberg1, Felix Keber1, Christian Cyron1, Jan Faix2, and Andreas Bausch1 — 1Technical University of Munich, Munich, Germany — 2Hannover Medical School, Hannover, Germany
Interactions between the cytoskeleton and the cell membrane are essential for various cellular processes. Here we reconstitute lamellipodia and filopodia like structures in a bottom up approach inside giant unilamellar vesicles (GUVs). We couple the network from the inside to the lipid membrane of GUVs. By the use of different actin binding proteins (ABPs) we control the binding characteristics of actin to the membrane and the network architecture. One of the key players is Arp2/3, which binds via its activator VCA to the vesicle's membrane. The autocatalytic nature of Arp2/3 and the finite volume of the vesicle result in the formation of patches of a dense actin network, which ultimately leads to pronounced membrane deformations. The capping protein (CP) determines thereby the resulting membrane deformations by finetuning the actin network geometry. We are able to model the formation of the localized networks by kinetic equations, a continuum elastic model suffices to describe the resulting membrane shape deformations.