Dresden 2017 – scientific programme

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BP: Fachverband Biologische Physik

BP 38: Membranes and Vesicles I

BP 38.8: Talk

Wednesday, March 22, 2017, 12:00–12:15, HÜL 386

High-speed single particle tracking on giant unilamellar vesicles — Susann Spindler, •Martin Kaller, and Vahid Sandoghdar — Max Planck Institute for the Science of Light, Erlangen, Germany

Interferometric scattering detection microscopy (iSCAT) is a powerful method for single particle tracking (SPT) experiments. Recently, we reported on the use of iSCAT for visualizing the diffusion of gold nanoparticles (GNPs) as small as 5nm attached to lipids in model membranes with nanometer lateral precision and at up to 1 MHz frame rate [1]. Here, we demonstrate one of the unique capabilities of iSCAT, namely high axial resolution in tracking the displacement of a nanoparticle and present three-dimensional trajectories of GNP-labeled lipids and unlabeled virus-like particles diffusing on a giant unilamellar vesicle (GUV) membrane. We discuss the differences in the observed diffusion behaviour in this system and compare them to our previous studies in supported bilayers [2].

[1] S. Spindler, J. Ehrig, K. König, T. Nowak, M. Piliarik, H. E. Stein, R. W. Taylor, E. Garanger, S. Lecommandoux, I. D. Alves, V. Sandoghdar, J. Phys. D: Appl. Phys. 49 (2016) [2] C.-L. Hsieh, S. Spindler, J. Ehrig, V. Sandoghdar, J. Phys. Chem. B 118 (2014).