Dresden 2017 – scientific programme
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BP: Fachverband Biologische Physik
BP 4: Systems Biology & Gene Expression and Signalling
BP 4.3: Talk
Monday, March 20, 2017, 15:45–16:00, ZEU 250
Magnetogenetic Manipulation of Intracellular Signalling — •Cornelia Monzel1, Chiara Vicario1, Domenik Lisse2, Mathieu Coppey1, Jacob Piehler2, and Maxime Dahan1 — 1Laboratoire Physico-Chimie, Institut Curie, CNRS UMR168, 75005 Paris, France — 2University of Osnabrück, Department of Biology, 49076 Osnabrück, Germany
Many cell functions rely on the coordinated activity of signalling pathways at a subcellular scale. However, there are few tools capable of probing and perturbing signalling networks with a spatial resolution matching the intracellular dimensions of their activity patterns. Here, we develop a generic magnetogenetic approach based on functionalized magnetic nanoparticles (MNPs) targeting an intracellular protein of interest. Upon protein recruitment the MNP-protein complexes act as nanoscopic hot spots that can be displaced by magnetic forces to provide molecularly graded information to the cell and to trigger a signal transduction pathway that brings about a cellular response. We demonstrate that magnetic nanoparticles based on the natural iron storage protein ferritin are ideally suited for intracellular applications. We use these ferritin MNP to manipulate Rho-GTPases - a set of molecular switches known to regulate cell morphology via complex spatiotemporal patterns of activity. The MNP-Rho-GTPase mediated stimulus is then shown to trigger morphological and signalling activity.