Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 48: Membranes and Vesicles II

BP 48.8: Vortrag

Donnerstag, 23. März 2017, 12:00–12:15, HÜL 386

Scattering Study on Small Unilamellar DMPC-Vesicles Incorporating the Saponin Escin — •Carina Dargel¹, Ramsia Sreij¹, Aurel Radulescu², and Thomas Hellweg¹ — ¹Physical and Biophysical Chemistry, Bielefeld University, Germany — ²Jülich Center for Neutron Science, outstation at FRM II, Garching, Germany

1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) belongs to the class of phospholipids and acts i.a. as a major membrane component. Therefore, model membranes consisting of DMPC mimic biological membranes quite well and allow to study effects of additives under different conditions, e.g. composition and temperature. Saponins are plant derived surfactants which occur among others in nuts and garlic and exhibit an amphiphilic structure built of a hydrophobic steroidic or triterpenic backbone with a varying number of hydrophilic sugar chains. The interaction of saponins with biological membranes is not yet scrutinized. Therefore in this study the effect of the pure saponin escin on small unilamellar vesicles of DMPC, prepared by extrusion, is investigated mainly by different scattering methods in dependence on the escin-amount and the temperature. An incorporation of escin above a critical amount can be deduced from the investigated parameters, namely the thermal phase transition temperature and vesicle size parameters like the radius, membrane thickness and lipid head-to-head distance within one monolayer.