Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 5: Single Molecule Biophysics

BP 5.1: Hauptvortrag

Montag, 20. März 2017, 15:00–15:30, HÜL 386

Metal Induced Energy Transfer — •Jörg Enderlein — 3. Physikalisches Institut, Georg-August-Universität, Friedrich-Hund-Platz 1, 37077 Göttingen

Classical fluorescence microscopy is limited in resolution by the wavelength of light (diffraction limit) restricting lateral resolution to ca. 200 nm, and axial resolution to ca. 500 nm. However, recent years have seen a tremendous development in super-resolution techniques, such as Photoactivatable Localization Microscopy (PALM) or STochastic Optical Reconstruction Microscopy (STORM), pushing the lateral resolution down to a few nanometers. However, even with these methods, the resolution along the optical axis is typically a factor 3 to 5 worse than the lateral resolution. Recently, we have developed a new method for localizing fluorescent emitters along the optical axis with nanometer accuracy. The method is based on the energy transfer of the excited fluorophore into surface plasmons of a thin metallic film, which is extremely sensitive on the distance between the fluorophore and the metal surface. We call this method Metal Induced Energy Transfer or MIET imaging. I will explain the physical foundations of MIET, and will present numerous of its applications.