Dresden 2017 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 55: Cell Migration and Contraction
BP 55.3: Vortrag
Donnerstag, 23. März 2017, 15:45–16:00, HÜL 386
Time-Resolved Measurement of Highly Dynamic Force Evolution in Small Cells — •Jana Hanke1, Dimitri Probst2, Ulrich S. Schwarz2, and Sarah Köster1 — 1Institute of X-Ray Physics, University of Göttingen, Göttingen, Germany — 2Institute for Theoretical Physics and BioQuant, Heidelberg University, Heidelberg, Germany
Force generation is an important aspect in numerous essential biological processes like rigidity sensing, spreading and motility. Measuring such forces as they are exerted on the cellular environment at high spatial resolution and in a time-resolved manner remains a challenge. Here we optimise traction force microscopy on soft elastic substrates for measurements of human blood platelets, which generate comparatively high traction forces although they are the smallest cells in the human body. By tracking force evolution over 30 minutes, we characterise the cells' highly dynamic force development on substrates of 19 to 83 kPa stiffness. Independent of the stiffness, we find three distinct behaviours in individual cells, namely platelets reaching a force plateau, as well as a relaxing or oscillatory behaviour after initial contraction. The average initial contraction velocity, however, is dependent of the stiffness as it increases with increasing stiffness.