Dresden 2017 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 6: Cell Mechanics (Joint Session BP/DY)
BP 6.2: Vortrag
Montag, 20. März 2017, 15:30–15:45, SCH A251
Feeling for Phenotype: Real-Time Deformability Cytometry for Label-Free Cell Functional Assays — •Oliver Otto1,2,3, Philipp Rosendahl1,3, Maik Herbig1, Angela Jacobi1,4, Martin Kräter1,4, Nicole Töpfner1, Marta Urbanska1, Maria Winzi1, Katarzyna Plak1, Alexander Mietke5, Christoph Herold3, Daniel Klaue3, Edwin Chilvers6, Reinhard Berner4, Martin Bornhäuser4, and Jochen Guck1 — 1Technische Universität Dresden, Dresden, Germany — 2Universität Greifswald, Greifswald, Germany — 3Zellmechanik Dresden, Dresden, Germany — 4Universitätsklinikum Dresden, Dresden, Germany — 5Max-Planck-Institut für Molekulare Zellbiologie und Genetik, Dresden, Germany — 6University of Cambridge, Cambridge, United Kingdom
By real-time deformability cytometry (RT-DC), we have introduced a high-throughput method for continuous mechanical single-cell classification of heterogeneous cell populations at rates of several hundred cells per second. Here, we demonstrate the extension of this method towards a multi-parameter biological assay where phenotyping is based on quantitative image analysis. Performing RT-DC on whole blood we highlight its potential to identify subsets in heterogeneous cell populations without any labelling and extensive sample preparation. We also demonstrate its capability to detect lineage-, source- and disease-specific mechanical phenotypes in primary human hematopoietic stem cells and mature blood cells. In summary, RT-DC enables marker-free, quantitative phenotyping of heterogeneous cell populations with a throughput comparable to standard flow cytometry.