Dresden 2017 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Posters - Bioimaging and Spectroscopy
BP 8.10: Poster
Montag, 20. März 2017, 17:30–19:30, P3
Beyond the beaten track: Pushing the limits of fluorescence microscopy — •Hannah S. Heil1, Benjamin Schreiber1, Si-Yun Liu1, Martin Kamp2, Markus Sauer3, and Katrin G. Heinze1 — 1Rudolf Virchow Center, Research Center for Experimental Biomedicine, University of Würzburg — 2Technische Physik, University of Würzburg, Am Hubland, 97074 Würzburg, Germany — 3Department of Biotechnology and Biophysics, Biozentrum, University of Würzburg
Combining optical with plasmonic approaches opens exciting perspectives for fluorescence microscopy: So called surface plasmons in specially designed nanostructures can generate extremely high photon densities in a nanoscopic volume that is much lower than the Abbe criteria usually allows. The interaction of fluorophores with plasmonic surfaces enables amplified fluorescence, increased photostability and distance dependent dynamical and spectral emission shifts. All of these effects are very welcome in pushing the two fundamental limits of fluorescence microscopy: contrast and resolution, particularly in the axial dimension. The strength of the approach is that - except for special cover glasses - no special microscope setup is required. Here we show that biocompatible plasmonic nanostructures fabricated on microscopy slides can improve the resolution of the super-resolution technique dSTORM by boosting the signal and thus the localization precision by a factor of two. Finally we give an outlook on how the plasmonic effects could allow 3D reconstructions of molecular distributions and interactions in live cells with nanometer precision.