Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 8: Posters - Bioimaging and Spectroscopy

BP 8.12: Poster

Montag, 20. März 2017, 17:30–19:30, P3

Network properties and dynamics of the endoplasmic reticulum — •Lorenz Stadler, Konstantin Speckner, and Matthias Weiss — University of Bayreuth, Bayreuth

The endoplasmic reticulum (ER) is an essential cellular organelle that assumes the shape of an extended network of interconnected membrane tubules and sheets. Being responsible for crucial cellular functions, e.g. protein folding and lipid synthesis, malfunctions of the ER are linked to severe diseases. To gain insights into the dynamic ER morphogenesis, we have used fluorescence imaging with high spatiotemporal resolution. ER images taken in cells at different states were skeletonized in a custom-made procedure, hence reducing the organelle's shape to planar graphs composed of edges and nodes. These graphs were analyzed via a set of network measures to quantify topological and geometrical features. Moreover, the motion of network nodes as well as the motion of membrane domains on the ER were analyzed in the presence and absence of cytoskeletal elements. These data highlight the role of active fluctuations for the ER's dynamic morphogenesis in the crowded interior of living cells.