Dresden 2017 – scientific programme

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BP: Fachverband Biologische Physik

BP 8: Posters - Bioimaging and Spectroscopy

BP 8.15: Poster

Monday, March 20, 2017, 17:30–19:30, P3

Investigating the temperature dependence of lipid mobility in free-standing membranes using circular scanning fluorescence correlation spectroscopy — •Araceli Sebastián, Jonas Mücksch, Philipp Blumhardt, Laura Kacenauskaite, Christian Niederauer, Eugene P. Petrov, and Petra Schwille — Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany

0pt nohyphenation Circular scanning Fluorescence Correlation Spectroscopy (csFCS) is a calibration-free alternative to conventional static point FCS [1,2]. Not only does csFCS allow for the direct determination of the diffusion coefficient, but, in case of quasi-planar membranes, also avoids potential errors originating from imprecise axial positioning. Previously, csFCS has been used to measure diffusion in giant unilamellar vesicles (GUVs), serving as a model of free-standing lipid membranes [3]. Despite the immanent importance of model membranes, their temperature-dependent lipid mobility has been subject to only few studies. Here, we report on our progress of employing csFCS to systematically characterize the diffusion in GUVs over a wide range of temperatures.

[1] K. M. Berland, P.T.C. So, Y. Chen, W.W. Mantulin, and E. Gratton, Biophys. J. 71, 410 (1996).

[2] Z. Petrášek and P. Schwille, Biophys. J. 94, 1437 (2008).

[3] Z. Petrášek, S. Derenko, and P. Schwille, Opt. Express 19, 25006 (2011).