Dresden 2017 – scientific programme
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BP: Fachverband Biologische Physik
BP 8: Posters - Bioimaging and Spectroscopy
BP 8.16: Poster
Monday, March 20, 2017, 17:30–19:30, P3
Super-Resolution by Structured-Illumination-Axial-Tomography (SIAT) — •Florian Schock1,2,3, Verena Richter4, Thomas Bruns4, Udo Birk1,3, Rainer Heintzmann5,6, Herbert Schneckenburger4, and Christoph Cremer1,2,3 — 1Institute of Molecular Biology, University of Mainz — 2Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg — 3Kirchhoff Institute for Physics, University of Heidelberg — 4Aalen University of Applied Sciences, Institute of Applied Research, Aalen, Germany — 5Institute for Physical Chemistry and Abbe Center of Photonics, University of Jena — 6Leibniz Institute of Photonic Technology, Jena, Germany
Since the first developments of super-resolution-microscopy (SRM), methods allowing to circumvent (break) the Abbe limit of optical resolution, a variety of different complementary SRM techniques were discovered. Due to differences in the mode of operation, a further optimization of super-resolution imaging can be achieved by appropriate combinatorial strategies. One of these is the combination of Structured-Illumination-Microscopy(SIM) and Micro-Axial-Tomography (AT). Both of them operate in the visible range spectra and are usable for live-cell imaging. While SIM allows for a resolution-improvement of a factor 2 in all spatial directions (3D), together with appropriate image processing, SIM-AT makes it possible to achieve a homogeneous effective resolution down to 100nm in all directions.