Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 8: Posters - Bioimaging and Spectroscopy

BP 8.18: Poster

Montag, 20. März 2017, 17:30–19:30, P3

A theoretical framework for spatiotemporal chemical imaging with nanosensors — •Daniel Meyer, Annika Hagemann, and Sebastian Kruss — Institute of Physical Chemistry, Göttingen University, Germany

Fluorescent nanosensors provide many beneficial properties and are often used to gain precise single-molecule data from biological system. The collective imaging of many sensors can, moreover, supply spatial and temporal information about the local concentration of a given analyte and thus is able to identify fast changing processes on a nanoscale. This idea of chemical imaging with nanosensors becomes attractive when studying chemical signaling between cells. We developed a theoretical framework to simulate the fluorescence image of arrays of nanosensors in response to a spatiotemporal concentration profile. We especially focus on the role of sensor kinetics as it determines how fast sensors can report about concentration changes. For that purpose, the (fluorescence) response of each single nanosensor is modeled with a Monte-Carlo simulation that describes the binding/debinding of the analyte and the respective fluorescence change. Multiple nanosensors are arranged on a surface and exposed to a concentration pattern c(x,y,t) of an analyte. We account for the Abbe-limit and the acquisition speed and resolution of the optical setup and determine the resulting sensor array response images I(x,y,t). Consequently we introduce terms for the spatial and temporal resolution and simulate phase diagrams that allow us to predict the best binding properties of our nanosensors for fast release events such as neurotransmitter releases.